Survival of grafts of three Pinus species with two techniques and two healing methods
DOI:
https://doi.org/10.29298/rmcf.v14i76.1315Abstract
Grafting is the most widely used vegetative propagation method in conifers to clone superior genotypes of forest species. The objective of the present study was to evaluate the effect of two grafting techniques (terminal cleavage and side veneer) and two healing methods (natural and application of healing wax) in three economically important species in Durango: Pinus engelmannii, P. cooperi, and P. durangensis. The experiment was established using a randomized complete block design with a 3×2×2 factorial arrangement and nine replications. Final survival was assessed at 180 days. The effect of individual treatments and their interactions was determined with Kruskal-Wallis, Dunn, and Dunnett tests. Significant differences (p<0.05) were obtained; the highest survival rate for P. engelmannii was 32.9 %, 16.3 % with side-veneer grafting, and 16.9 % with the use of healing wax. The double interactions between species and grafting technique, and between species and healing method showed significant differences (p<0.05), in addition to the triple interaction. The highest survival values were observed in side-veneer and terminal-cleft grafts of P. engelmannii, with 40 and 25.8 %, respectively, as well as in the grafts of P. engelmannii with wax application, whose value was 43.3 %. The best triple interaction was that of side-veneer grafts of P. engelmannii with healing wax (50 %). P. engelmannii responded best to grafting. For P. cooperi and P. durangensis, it is recommended to search for alternative grafting techniques.
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Almqvist, C. 2013. Survival and strobili production in topgrafted scions from young Pinus sylvestris seedlings. Scandinavian Journal of Forest Research 28(6):533-539. Doi: 10.1080/02827581.2013.803598. DOI: https://doi.org/10.1080/02827581.2013.803598
Aparicio-Rentería, A., H. Viveros-Viveros y V. Rebolledo-Camacho. 2013. Huertos semilleros clonales: una alternativa para los programas de reforestación en Veracruz. Revista Mexicana de Ciencias Forestales 4(20):90-97. Doi: 10.29298/rmcf.v4i20.373. DOI: https://doi.org/10.29298/rmcf.v4i20.373
Barrera-Ramírez, R., J. J. Vargas-Hernández, R. López-Aguillón, H. J. Muñoz-Flores, E. J. Treviño-Garza and O. A. Aguirre-Calderón. 2021. Impact of external and internal factors on successful grafting of Pinus pseudostrobus var. oaxacana (Mirov) Harrison. Revista Chapingo Serie Ciencias Forestales y del Ambiente 27(2):243-256. Doi: 10.5154/r.rchscfa.2020.05.037. DOI: https://doi.org/10.5154/r.rchscfa.2020.05.037
Blada, I. and T. Panea. 2011. Improvement of grafting procedures for the ornamental species: I. Picea pungens Engelm. var. glauca Regel. Annals of Forest Research 54(2):185-196. https://www.afrjournal.org/index.php/afr/article/viewFile/89/135. (21 de octubre de 2022).
Blada, I. and T. Panea. 2012. Improvement of grafting procedures for the ornamental species: II. Abies concolor [(Gord. & Glend.) Lindl]. Annals of Forest Research 55(1):25-31. https://www.afrjournal.org/index.php/afr/article/viewFile/72/107. (21 de octubre de 2022).
Castro-Garibay, S. L., Á. Villegas-Monter, J. López-Upton, M. Sandoval-Villa and L. Arévalo-Galarza. 2022. Effective protocol to increase the percentage of grafting success of Pinus greggii Engelm. var. australis Donahue et López. Revista Chapingo Serie Ciencias Forestales 28(2):225-240. Doi: 10.5154/r.rchscfa.2021.03.014. DOI: https://doi.org/10.5154/r.rchscfa.2021.03.014
Chávez I., C. 2016. Injertado de corona en tres especies del género Pinus en la región de el Salto, Pueblo Nuevo, Durango. Tesis de Maestría. Facultad de Ciencias Forestales. Universidad Juárez del Estado de Durango. Durango, Dgo., México. 68 p.
Cuevas C., J. C. 2014. Propagación Vegetativa de Pinus leiophylla Schiede ex Schltdl. et Cham. Tesis de Maestría. Postgrado en Ciencias Forestales. Colegio de Postgraduados, Campus Montecillo. Texcoco, Edo. Méx., México. 72 p.
García A., A. y M. S. González E. 2003. Pináceas de Durango. Instituto de Ecología, A.C. Xalapa, Ver., México. 179 p.
González-Jiménez, B., M. Jiménez-Casas, J. López-Upton, M. Á. López-López and R. Rodríguez-Laguna. 2022. Combination of grafting techniques to clone superior genotypes of Pinus patula Schiede ex Schltdl. et Cham. Agrociencia 56(5):1-12. Doi: 10.47163/agrociencia.v56i5.2582. DOI: https://doi.org/10.47163/agrociencia.v56i5.2582
Hibbert-Frey, H., J. Frampton, F. A. Blazich, D. Hundley and E. Hinesley. 2011. Grafting fraser fir (Abies fraseri): effect of scion origin (crown position and branch order). HortScience 46(1):91-94. Doi: 10.21273/HORTSCI.46.1.91. DOI: https://doi.org/10.21273/HORTSCI.46.1.91
Martínez S., M. y J. Á. Prieto R. 2011. Determinación de áreas potenciales para el establecimiento de plantaciones comerciales forestales en la región norte de México. Folleto Técnico Núm. 47. INIFAP-Campo Experimental Valle del Guadiana. Durango, Dgo., México. 35 p.
McKeand, S. E., J. B. Jett, J. R. Sprague and M. N. Todhunter. 1987. Summer wax grafting of loblolly pine. Southern Journal of Applied Forestry 11(2):96-99. Doi: 10.1093/sjaf/11.2.96. DOI: https://doi.org/10.1093/sjaf/11.2.96
Muñoz F., H. J., J. Á. Prieto R., A. Flores G., T. Pineda O. y E. Morales G. 2013. Técnicas de injertado “enchapado lateral” y “fisura terminal” en Pinus pseudostrobus Lindl. Folleto Técnico Núm. 68. INIFAP-Campo Experimental Valle del Guadiana. Durango, Dgo., México. 48 p.
Pérez-Luna, A., J. Á. Prieto-Ruíz, J. López-Upton, A. Carrillo-Parra, … and J. C. Hernández-Díaz. 2019. Some factors involved in the success of side veneer grafting of Pinus engelmannii Carr. Forests 10(2):112-229. Doi: 10.3390/f10020112. DOI: https://doi.org/10.3390/f10020112
Pérez-Luna, A., C. Whenkel, J. Á. Prieto-Ruíz, J. López-Upton, … and J. C. Hernández-Díaz. 2020a. Grafting in conifers: A review. Pakistan Journal of Botany 52(4):1369-1378. Doi: 10.30848/PJB2020-4(10). DOI: https://doi.org/10.30848/PJB2020-4(10)
Pérez-Luna, A., C. Wehenkel, J. Á. Prieto-Ruíz, J. López-Upton and J. C. Hernández-Díaz. 2020b. Survival of side grafts with scions from pure species Pinus engelmannii Carr. and the P. engelmannii × P. arizonica Engelm. var. arizonica hybrid. PeerJ 8: e8468. Doi: 10.7717/peerj.8468. DOI: https://doi.org/10.7717/peerj.8468
Pérez-Luna, A., J. C. Hernández-Díaz, C. Wehenkel, S. L. Simental-Rodríguez, J. Hernández-Velasco and J. Á. Prieto-Ruíz. 2021. Graft survival of Pinus engelmannii Carr. in relation to two grafting techniques with dormant and sprouting buds. PeerJ 9:e12182. Doi: 10.7717/peerj.12182. DOI: https://doi.org/10.7717/peerj.12182
R Core Team. 2020. The R Project for Statistical Computing (versión 3.5.3). Vienna, Austria. The R foundation for statistical computing. https://www.R-project.org/. (21 de octubre de 2022).
Rosier, C. L., J. Frampton, B. Goldfarb, F. C. Wise and F. A. Blazich. 2005. Stumping height, crown position, and age of parent tree influence rooting of stem cuttings of Fraser fir. HortScience 40(3):771-777. Doi: 10.21273/HORTSCI.40.3.771. DOI: https://doi.org/10.21273/HORTSCI.40.3.771
Solorio-Barragán, E. R., P. Delgado-Valerio, A. Molina-Sánchez, V. Rebolledo-Camacho and M. Á. Tafolla-Martínez. 2021. Interspecific grafting as an alternative for asexual propagation of Pinus rzedowskii Madrigal & Caball. Del. in danger extinction. Revista Chapingo Serie Ciencias Forestales y del Ambiente 27(2):277-288. Doi: 10.5154/r.rchscfa.2020.06.046. DOI: https://doi.org/10.5154/r.rchscfa.2020.06.046
Stewart, J. F., R. Will, B. S. Crane and D. Nelson. 2017. Occurrence of shortleaf x loblolly pine hybrids in shortleaf pine orchards: implications for ecosystem restoration. Forest Science 63(2):225-231. Doi: 10.5849/forsci.15-167. DOI: https://doi.org/10.5849/forsci.15-167
Ungureanu, S., T. Panea and V. Ungureanu. 2013. Mijloace noi pentru creştere randamentului şi îmbunătăţirea calităţii materialului săditor de măr prin altoirea la masă. Horticultură, Viticultură şi vinificaţie, Silvicultură şi grădini publice, Protecţia plantelor 36(1):30-33. https://ibn.idsi.md/sites/default/files/imag_file/30-33_10.pdf. (21 de octubre de 2022).
White, G., W. J. Lowe and J. Wright. 1983. Paraffin grafting techniques for loblolly pine. Southern Journal of Applied Forestry 7(3):116-118. Doi: 10.1093/sjaf/7.3.116. DOI: https://doi.org/10.1093/sjaf/7.3.116
Yuan, H., S. Niu, X. Zhou, Q. Du, Y. Li and W. Li. 2016. Evaluation of seed production in a first-generation seed orchard of Chinese pine (Pinus tabuliformis). Journal of Forestry Research 27(5):1003-1008. Doi: 10.1007/s11676-016-0238-x. DOI: https://doi.org/10.1007/s11676-016-0238-x
Zobel, B. and J. Talbert. 1984. Applied forest tree improvement. John Wiley. New York, NY, United States. 505 p.
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