Leaf morpho-anatomical characterization in Pinus patula Schiede ex Schltdl. & Cham. progenies
DOI:
https://doi.org/10.29298/rmcf.v17i93.1597Keywords:
Needles, leaf anatomy, resin canals, endoderm, epiderm, hypodermAbstract
Progeny trials are experimental fields where the genetics of selected parent trees are evaluated. They bring together families from different origins that vary due to genetic and/or environmental factors. The objective of this research was to describe and analyze the foliar morpho-anatomy of Pinus patula from two progeny trials established at the Tlatoxca ranch in the state of Hidalgo and the Ojo de Agua ejido in the state of Veracruz, Mexico. Ten fascicles were collected from 64 trees. Cross-sections were made and stained with safranin and alcian blue. The structures were analyzed and described using an optical binocular microscope. The number of needles per fascicle, the number of stomatal rows (abaxial and adaxial surfaces), and the number of resin canals were subjected to an analysis of variance (P≤0.05). A single-layered epidermis composed of highly clarified rectangular cells was observed. The hypodermis is single-layered and bi-layered; 54 % of the needles at the Tlatoxca ranch exhibited a mostly bi-layered hypodermis, while at the Ojo de Agua ejido, 33 % did. Significant differences were found in the number of resin canals (P≤0.0001); at the Tlatoxca ranch, the number ranged from (2-)3(-6) with an average of 3.48, while at the Ojo de Agua ejido, the range was (0-)3(-4), with an average of 2.64. The resin canals were frequently located medially and internally, and exceptionally externally and septally. The environmental conditions of each plantation site influenced some morpho-anatomical characteristics of the P. patula needles in two two-year-old plantations.
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