Assessment of culture media in the in vitro multiplication of mahogany (Swietenia macrophylla King)
DOI:
https://doi.org/10.29298/rmcf.v17i93.1583Keywords:
Mahogany, culture media, micropropagation, Generalized Linear Model, multiplication, Swietenia macrophylla KingAbstract
Swietenia macrophylla is a species of high economic value and has been subject to high timber extraction over decades. It is included in Appendix II of the Convention on International Trade in Endangered Species of Wild Fauna and Flora (Cites). Its low germination rate and the lack of genotypic and phenotypic selection programs hinder its propagation, resulting in heterogeneous plantations. Thus, plant tissue culture is an option for multiplying forest species. In this study, Murashige and Skoog (MS) and Woody Plant Medium (WPM), both supplemented with 2 mg L-1 of 6-benzylaminopurine (BAP), were evaluated as a reproducible method to facilitate the multiplication of mahogany plants, focusing on shoot length and number of nodes. The results indicate that the interaction between the culture medium and the BAP concentration influences both variables. In MS medium, shoot length and the number of nodes decreased with increasing BAP concentration from 0 to 2 mg L-1, while the opposite occurred in WPM medium. For multiplication (each node giving rise to a new plant), MS medium without growth regulators produced shoots of 11.18 mm with an average of 4.08 nodes, resulting in a multiplication rate of 4.08. WPM medium, on the other hand, produced shoots of 11.82 mm with an average of 3.29 nodes. These results suggest that in vitro multiplication via nodes (buds) in a medium without growth regulators is possible.
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