De novo establishment of an in vitro cell line culture derived from the intestine of sawflies (Hymenoptera: Diprionidae)
DOI:
https://doi.org/10.29298/rmcf.v13i69.1088Keywords:
Biological control, cellular culture, epithelial cells, Nuclear Polyhedrosis Virus, in vitro culture, intestinal epithelialAbstract
Pines, cedars and oaks forests are affected by different arthropod pests, one of which are the wasps of the family Diprionidae commonly known as sawflies. Swarms of these organisms cause defoliation on large extensions of pines and oaks along the national territory, and there is an urgent need to implement measures for the control of sawflies. One successful measure applied in other countries with similar forest pest control issues is the use of specific baculoviruses for the control of Diprionidae wasps, which has proved to be effective in the biological control of sawflies by infecting the larval stage of theses wasps, and thus increasing their mortality. However, there are no current projects in México that evaluate the identification and isolation of gamma-baculovirus destined to the biological control of forest pests. Our study addresses the technical difficulties of obtaining an intestinal cell line from Diprionidae sawflies, and we report the isolation and in vitro culture of intestinal cells.
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