IMPACTO DEL OZONO SOBRE EL POLEN DE Pinus hartwegii Lindl.

Authors

  • Tomás Hernández Tejeda Centro Nacional de Investigación Disciplinaria en Conservación y Mejoramiento de Ecosistemas Forestales (CENID-COMEF), INIFAP. Ave. Progreso No. 5, Viveros de Coyoacán 0411 O México, 2 D.F.
  • Roger M. Cox Canadian Forest Service-Maritimes, Natural Resources Ganada
  • John Malcolm Canadian Forest Service-Maritimes, Natural Resources Ganada
  • María de Lourdes de la Isla de Bauer Colegio de de Postgraduados. Postgraduados. Programa Programa de Forestal. Hidrociencias
  • Jesús Vargas Hernández Colegio de Postgraduados. Programa Forestal
  • Alejandro Velázquez Martínez Colegio de Postgraduados. Programa Forestal
  • Dionicio Alvarado Rosales Colegio de Postgraduados. Programa de Fitopatología

Keywords:

Fumigation, pollen germination, ozone, Pinus hartwegii, pollen, pollinic tube

Abstract

Air pollution, particularly photochemical oxidants, induces changes in the reproductive processes of vegetative species In this study, the effect of ozone on germination and tube growth of Pinus hartwegii pollen grains was investigated. Pollen was collected from 3 forest areas in the Mexico City Air Basin with varying air pollution exposure levels: Desierto de los Leones, Ajusco and Zoquiapan. lt was suspended in tissue culture media (Brewbaker solution) plus a solidifying agent (Gelrite) ata concentration near 2,000 grains per 50 µI droplet. Droplets were deposited on glass slides, which were incubated in Petri dishes that served as humidity chambers. The Petri dishes were opened daily only during the periods of ozone fumigation. The pine pollen was artificially exposed in special fumigation chambers to: 0.03 (control), 0.20 and 0.30 ppm ozone for 3 hours per day, for 3 or 6 consecutive days. Results showed that pollen grains were equally affected by ozone, in spite of theír site of origin. The inhibition of germination and tube growth of P. hartwegii pollen was greater at higher ozone concentrations, and pollen germination decreased significantly ozone exposure periods were longer.

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Published

2020-10-16

How to Cite

Hernández Tejeda, Tomás, Roger M. Cox, John Malcolm, María de Lourdes de la Isla de Bauer, Jesús Vargas Hernández, Alejandro Velázquez Martínez, and Dionicio Alvarado Rosales. 2020. “IMPACTO DEL OZONO SOBRE EL POLEN DE Pinus Hartwegii Lindl”. Revista Mexicana De Ciencias Forestales 26 (89). México, ME:5-18. https://cienciasforestales.inifap.gob.mx/index.php/forestales/article/view/916.