IMPACTO DEL OZONO SOBRE EL POLEN DE Pinus hartwegii Lindl.
Keywords:
Fumigation, pollen germination, ozone, Pinus hartwegii, pollen, pollinic tubeAbstract
Air pollution, particularly photochemical oxidants, induces changes in the reproductive processes of vegetative species In this study, the effect of ozone on germination and tube growth of Pinus hartwegii pollen grains was investigated. Pollen was collected from 3 forest areas in the Mexico City Air Basin with varying air pollution exposure levels: Desierto de los Leones, Ajusco and Zoquiapan. lt was suspended in tissue culture media (Brewbaker solution) plus a solidifying agent (Gelrite) ata concentration near 2,000 grains per 50 µI droplet. Droplets were deposited on glass slides, which were incubated in Petri dishes that served as humidity chambers. The Petri dishes were opened daily only during the periods of ozone fumigation. The pine pollen was artificially exposed in special fumigation chambers to: 0.03 (control), 0.20 and 0.30 ppm ozone for 3 hours per day, for 3 or 6 consecutive days. Results showed that pollen grains were equally affected by ozone, in spite of theír site of origin. The inhibition of germination and tube growth of P. hartwegii pollen was greater at higher ozone concentrations, and pollen germination decreased significantly ozone exposure periods were longer.
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