Overexpression of PtCSP4 in poplar promotes greater potential for PCB phytoremediation
DOI:
https://doi.org/10.29298/rmcf.v12i65.781Keywords:
Poplar, Arabidopsis, aroclor, in vitro, PtCSP4, Populus trichocarpa Torr. A. & Gray ex Hook., toleranceAbstract
Phytoremediation is a widely accepted biotechnology that reduces, absorbs and degrades toxic pollutants. Polychlorinated biphenyls (PCB) are persist organic pollutants that cause harmful effects to the environment. It has been acknowledged that poplar (Populus trichocarpa) is fast growing, it has high transpiration and a complete genome sequence. Thus, it is considered a perfect model in phytoremediation. Genetically modified plants can become more efficient remediation sources of soil and water contaminated sites with polychlorinated biphenyls (PCB). Yb-1 is a multifunctional protein involved in the regulation of transcription, translation, mARN splicing and ADN repair. Yb1 transcription factor, is known in humans and mammals but very little in plants. Yb-1 in plants is best known as CSP (cold shock proteins). In this work, PtCSP4 (Potri.004G172600) gene was isolated and amplified from poplar. The gateway recombination system was used to clone PtCSP4 in a binary vector with a constitutive promoter. PtCSP4 was introduced in Arabidopsis thaliana and its tolerance was tested in presence of PCB (Aroclor 1221). Eight transgenic lines of in vitro cultured Arabidopsis were exposed to PCBs. All results suggest that PtCSP4 is a good candidate for phytoremediation of PCB.
Downloads
Published
How to Cite
Issue
Section
License
The authors who publish in Revista Mexicana de Ciencias Forestales accept the following conditions:
In accordance with copyright laws, Revista Mexicana de Ciencias Forestales recognizes and respects the authors’ moral right and ownership of property rights which will be transferred to the journal for dissemination in open access.
All the texts published by Revista Mexicana de Ciencias Forestales –with no exception– are distributed under a Creative Commons License Attribution-NonCommercial 4.0 International (CC BY-NC 4.0), which allows third parties to use the publication as long as the work’s authorship and its first publication in this journal are mentioned
The author(s) can enter into independent and additional contractual agreements for the nonexclusive distribution of the version of the article published in Revista Mexicana de Ciencias Forestales (for example, include it into an institutional repository or publish it in a book) as long as it is clearly and explicitly indicated that the work was published for the first time in Revista Mexicana de Ciencias Forestales.
For all the above, the authors shall send the form of Letter-transfer of Property Rights for the first publication duly filled in and signed by the author(s). This form must be sent as a PDF file to: ciencia.forestal2@inifap.gob.mx
This work is licensed under a Creative Commons Attribution-Noncommercial 4.0 International license.
