REGENERATION OF Paulownia elongata Steud. PLANTS BY DIRECT ORGANOGENESIS

Authors

  • Carlos R. Castillo-Martínez Centro Nacional de Recursos Genéticos. INIFAP
  • Ma. Alejandra Gutiérrez-Espinosa Especialidad de Genética, Colegio de Postgraduados
  • Marco T. Buenrostro-Nava specialidad de Genética, Colegio de Postgraduados
  • Víctor Manuel Cetina Alcalá Especialidad de Genética, Colegio de Postgraduados
  • Jorge Cadena Iñiguez Especialidad de Genética, Colegio de Postgraduados

DOI:

https://doi.org/10.29298/rmcf.v3i10.528

Keywords:

6-Benciladenine, naphtalenacetic acid, explants, micropropagation, Paulownia elongate Steud., forest plantations

Abstract

Paulownia elongata is an oriental fast-growing forest species that was introduced in Mexico at the end of 1998, as an option for wood commercial plantations, since its timber is light, which favours its use in furniture manufacturing, handicrafts, music instruments and in interior finish. The aim of this work was to determine the best in vitro culture conditions for plant regeneration of Paulownia elongata through several types of explants from steam segments (internode), leaves and petiole, which resulted in a protocol for direct organogenesis that can be used in genetic transformation systems. In all cases, to the basal MS medium were added 6-benzyladenine (BA) from 5 mg L-1 to 1.0 mg L-1 and naphtalenacetic acid (NAA) at different concentrations. Response was obtained in all of the explants with the 5 mg L-1 BA and 1.0 mg L-1 NAA combination; however internodal segments showed a better bud induction, by producing 83% explants with shoots and 1.52 shoots per explant with the 4 mg L-1 BA and 0.2 mg L-1 NAA combination, in contrast to those of petiole segments that produced their best results as 53% explants with shoots and 0.62 shoots per explant, with a 7 mg L-1 BA and 0.2 mg L-1 NAA mix.

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Published

2019-03-06

How to Cite

Castillo-Martínez Carlos R., Gutiérrez-Espinosa Ma. Alejandra, Marco T. Buenrostro-Nava, Cetina Alcalá Víctor Manuel, and Cadena Iñiguez Jorge. 2019. “REGENERATION OF Paulownia Elongata Steud. PLANTS BY DIRECT ORGANOGENESIS”. Revista Mexicana De Ciencias Forestales 3 (10). México, ME:41-49. https://doi.org/10.29298/rmcf.v3i10.528.

Issue

Vol. 3 No. 10 (2012)

Section

Scientific article

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