Effects of Trichoderma and Bacillus pre-treatments on the germination of Agave victoriae-reginae SEEDS T. Moore
DOI:
https://doi.org/10.29298/rmcf.v13i69.844Keywords:
Agave, endangered species, dormancy, noha, germinative treatment, seed viabilityAbstract
Application of germinative treatments is key to improve germination rates of forest species seeds, among which is found the use of microorganisms. In this study, the percentage of germination of Agave victoriae-reginae seeds treated with Trichoderma spp, and Bacillus spp was evaluated. Three treatments were tested: T1 (Trichoderma), T2 (Bacillus), and T3 (control), with three replications of 100 seeds each one. The seeds were immersed in a solution 1 × 106 CFU (treatments T1 and T2), and water in T3, then they were sowed, and the germination percentage was recorded daily. The germination began 5 days before the sown, which indicated that seeds did not present dormancy. Between 8° and 12° day an accelerated increase of germination was recorded in the three cases, until it ended at day 26, with 85 % as total germination for seeds treated with Trichoderma spp., 86.7 % with Bacillus spp, and 74 % with control. A significant effect of treatment on the germination percentage was found; even the use of both microorganisms accelerated the germination process compared to control. These results suggest that the use of Trichoderma spp, and Bacillus spp. as pregerminative treatments can improve the germination of A. victoriae-reginae and its long-term conservation, which contributes to the preservation of this endangered species.
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