Identification of the causal agent of antracnosis in the cultivation of the rubber tree [Hevea brasiliensis (Willd. ex A. Juss.) Müll. Arg.]

Authors

  • Adriana Rosalía Gijón Hernández Centro Nacional de Investigación Disciplinaria en Conservación y Mejoramiento de Ecosistemas Forestales, INIFAP
  • Iris Marley Pérez-Gálvez Centro Nacional de Investigación Disciplinaria en Conservación y Mejoramiento de Ecosistemas Forestales, INIFAP
  • Brenda Torres Huerta Centro Nacional de Investigación Disciplinaria en Conservación y Mejoramiento de Ecosistemas Forestales, INIFAP
  • Hilda Victoria Rojas-Silva Colegio de Postgraduados, Campus Montecillo
  • Elías Ortíz Cervantes Campo Experimental El Palmar, CIR-Golfo Centro, INIFAP

DOI:

https://doi.org/10.29298/rmcf.v10i56.551

Keywords:

Antracnose, phylogenetic, phytopathogen, fungus, rubber, PCR

Abstract

In 2016, a disease developed in clonal rubber tree gardens (clone IAN-710) in San Juan Bautista Tuxtepec, Oaxaca, the symptoms were observed in new leaves, buds and stems characterized by small watery spots that become in circular to irregular necrotic lesions, dark cream to black with yellow edges. Symptomatic samples were taken and 17 colonies were isolated in PDA media, seven from the stem and 10 from the leaves. The pathogen was determined using traditional techniques, PCR-Sequencing with the pair of ITS5 and ITS4 primers that amplified a 550 bp fragment. The phylogenetic analysis was performed with Bayesian inference with 1 000 000 generations and a final standard deviation of 0.008. The isolates presented morphological characteristics of the Colletotrichum genus, while phylogenetic analyzes indicated that the isolates were grouped within the species of the Colletotrichum gloeosporioides complex.

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Published

2019-12-05

How to Cite

Gijón Hernández, Adriana Rosalía, Iris Marley Pérez-Gálvez, Brenda Torres Huerta, Hilda Victoria Rojas-Silva, and Elías Ortíz Cervantes. 2019. “Identification of the Causal Agent of Antracnosis in the Cultivation of the Rubber Tree [Hevea Brasiliensis (Willd. Ex A. Juss.) Müll. Arg.]”. Revista Mexicana De Ciencias Forestales 10 (56). México, ME. https://doi.org/10.29298/rmcf.v10i56.551.

Issue

Vol. 10 No. 56 (2019)

Section

Scientific article

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